SIZE
0.1 mg
INTRODUCTION
Angiotensin-converting enzyme 2 (ACE-2) consists of 805 amino acids, including an N-terminal signal peptide a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail.
ACE2 can serve as the receptor for SARS-CoV and SARS-CoV-2 viruses. Binding of the S1 subunit of the SARS-CoV-2 Spike protein to the ACE2 triggers viral entry into the host cell. Therefore, the interaction between SARS-CoV-2 and ACE2 has become an area of interest to develop therapeutic treatments to fight against COVID-19.
DESCRIPTION
A DNA sequence encoding the signal peptide (Met1-Ala17) and extracellular domain of human ACE2 (Gln18-Ser740) (Uniprot No. Q9BYF1) was expressed with a human IgG1 Fc tag at the C-terminus linked by a flexible linker (GGGGS)2. The predicted molecular mass of ACE2 is 112 kDa for monomer and 224 kDa for dimer. The concentration of protein was determined by BCA.
SIZE
0.1 mg
INTRODUCTION
Angiotensin-converting enzyme 2 (ACE-2) consists of 805 amino acids, including an N-terminal signal peptide a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail.
ACE2 can serve as the receptor for SARS-CoV and SARS-CoV-2 viruses. Binding of the S1 subunit of the SARS-CoV-2 Spike protein to the ACE2 triggers viral entry into the host cell. Therefore, the interaction between SARS-CoV-2 and ACE2 has become an area of interest to develop therapeutic treatments to fight against COVID-19.
DESCRIPTION
A DNA sequence encoding the signal peptide (Met1-Ala17) and extracellular domain of human ACE2 (Gln18-Ser740) (Uniprot No. Q9BYF1) was expressed with a human IgG1 Fc tag at the C-terminus linked by a flexible linker (GGGGS)2. The predicted molecular mass of ACE2 is 112 kDa for monomer and 224 kDa for dimer. The concentration of protein was determined by BCA.
