SIZE
0.1 mg
INTRODUCTION TO THE MOLECULE
The spike (S) glycoprotein of SARS-CoV-2 is a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large scale. Here, we used plasmid with six beneficial proline substitutions modification to generate HexaPro Spike protein with better stability. Using cryo-EM, the structure of HexaPro spike protein is confirmed to retain the prefusion spike confirmation. Since S protein mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2), a stabilized prefusion spike protein can be used for the development of serological diagnostics and vaccines for SARS-CoV-2.
SIZE
0.1 mg
INTRODUCTION TO THE MOLECULE
The spike (S) glycoprotein of SARS-CoV-2 is a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large scale. Here, we used plasmid with six beneficial proline substitutions modification to generate HexaPro Spike protein with better stability. Using cryo-EM, the structure of HexaPro spike protein is confirmed to retain the prefusion spike confirmation. Since S protein mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2), a stabilized prefusion spike protein can be used for the development of serological diagnostics and vaccines for SARS-CoV-2.
