SIZE
96 wells/kit
INTRODUCTION
Insulin is a hormone synthesized by the b cells of pancreatic islets. It consists of two amino acid chains, A chain and B chain, linked with a sulphide bond. The A chain is made of 21 amino acids and B chain with 30 amino acids. Insulin has a molecular weight of 5.8KDa.
PRINCIPLE OF THE ASSAY
This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
SIZE
96 wells/kit
INTRODUCTION
Insulin is a hormone synthesized by the b cells of pancreatic islets. It consists of two amino acid chains, A chain and B chain, linked with a sulphide bond. The A chain is made of 21 amino acids and B chain with 30 amino acids. Insulin has a molecular weight of 5.8KDa.
PRINCIPLE OF THE ASSAY
This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
