SIZE
96 wells/kit
INTRODUCTION
PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.
PRINCIPLE OF THE ASSAY
This assay is a quantitative sandwich ELISA. The microtiter plate is pre coated with affinity purified antibody against mouse PM20D1. Standards and samples are pipetted into the wells and any human PM20D1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase biotin-labelled polyclonal antibody specific for mouse PM20D1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-horseradish peroxidase (STP-HRP) conjugate is added. After the last wash step, an HRP-substrate(TMB) solution is added and colour develops in proportion to the amount of mouse PM20D1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse PM20D1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
SIZE
96 wells/kit
INTRODUCTION
PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.
PRINCIPLE OF THE ASSAY
This assay is a quantitative sandwich ELISA. The microtiter plate is pre coated with affinity purified antibody against mouse PM20D1. Standards and samples are pipetted into the wells and any human PM20D1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase biotin-labelled polyclonal antibody specific for mouse PM20D1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-horseradish peroxidase (STP-HRP) conjugate is added. After the last wash step, an HRP-substrate(TMB) solution is added and colour develops in proportion to the amount of mouse PM20D1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse PM20D1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
