SIZE
2000 tests/box
INTRODUCTION
HbA1c is the term known as glycated hemoglobin. Throughout the whole life cycle of red blood cell, HbA1c is formed continuously by the adduction of glucose to the N-terminal of the hemoglobin beta chain. Formation of the sugar-Hb linkage indicates the presence of excessive sugar in the bloodstream, often indicative of diabetes. Therefore, measurement of %HbA1c can also be used to determine three-month average blood sugar level since the average lifespan of a red blood cell is four months. HbA1c level in diabetic subjects will be elevated by 2-3 fold over the levels found in normal individuals. The cut-off value for %HbA1c is 6.5%. The higher the %HbA1c, the higher chance the diabetic subjects are going to develop complications.
HbA1c PETIA kit developed by IMD can accurately measure %HbA1c in human whole blood sample.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of %HbA1c in human whole blood. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. Total hemoglobin and HbA1c have the same unspecific absorption rate to latex particles. After a short incubation, the test reagent R2 is added into the cuvette and mixed. Latex-HbA1c-mouse anti-human HbA1c antibody complex is formed. Agglutination is formed when goat anti-mouse IgG polyclonal antibody interacts with the monoclonal antibody. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The %HbA1c in unknown samples can be interpolated from a reference curve using the standards provided.
SIZE
2000 tests/box
INTRODUCTION
HbA1c is the term known as glycated hemoglobin. Throughout the whole life cycle of red blood cell, HbA1c is formed continuously by the adduction of glucose to the N-terminal of the hemoglobin beta chain. Formation of the sugar-Hb linkage indicates the presence of excessive sugar in the bloodstream, often indicative of diabetes. Therefore, measurement of %HbA1c can also be used to determine three-month average blood sugar level since the average lifespan of a red blood cell is four months. HbA1c level in diabetic subjects will be elevated by 2-3 fold over the levels found in normal individuals. The cut-off value for %HbA1c is 6.5%. The higher the %HbA1c, the higher chance the diabetic subjects are going to develop complications.
HbA1c PETIA kit developed by IMD can accurately measure %HbA1c in human whole blood sample.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of %HbA1c in human whole blood. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. Total hemoglobin and HbA1c have the same unspecific absorption rate to latex particles. After a short incubation, the test reagent R2 is added into the cuvette and mixed. Latex-HbA1c-mouse anti-human HbA1c antibody complex is formed. Agglutination is formed when goat anti-mouse IgG polyclonal antibody interacts with the monoclonal antibody. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The %HbA1c in unknown samples can be interpolated from a reference curve using the standards provided.
