SIZE
2000 tests/box
INTRODUCTION
Adiponectin (ADPN), also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue (fat). Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplementation of adiponectin protein can decrease blood glucose, improve insulin sensitivity, alleviate fatty liver and prevent atherosclerosis1. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), and decreased insulin sensitivity. A large number of longitudinal studies in different ethnic groups uniformly demonstrated that circulating adiponectin levels are decreased significantly in type 2 diabetes (T2D) and related complications and low circulating adiponectin levels indicate the increased risk for the development of T2D2. It is generally accepted that the higher the level of adiponectin, the lower the risk of developing diabetes. The level less than 4 mg/L in female or less than 3.5 mg/L in male indicates significant increased risk of developing diabetes.
Adiponectin PETIA kit developed by IMD takes advantage of its patented proprietary recombinant antigen and antibodies, and can accurately measure adiponectin in serum/plasma using any automatic chemical analyser.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of adiponectin in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with an antibody highly specific to adiponectin, is added into the cuvette and mixed. The presence of adiponectin in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of adiponectin in unknown samples can be interpolated from a reference curve using the standards provided.
SIZE
2000 tests/box
INTRODUCTION
Adiponectin (ADPN), also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue (fat). Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplementation of adiponectin protein can decrease blood glucose, improve insulin sensitivity, alleviate fatty liver and prevent atherosclerosis1. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), and decreased insulin sensitivity. A large number of longitudinal studies in different ethnic groups uniformly demonstrated that circulating adiponectin levels are decreased significantly in type 2 diabetes (T2D) and related complications and low circulating adiponectin levels indicate the increased risk for the development of T2D2. It is generally accepted that the higher the level of adiponectin, the lower the risk of developing diabetes. The level less than 4 mg/L in female or less than 3.5 mg/L in male indicates significant increased risk of developing diabetes.
Adiponectin PETIA kit developed by IMD takes advantage of its patented proprietary recombinant antigen and antibodies, and can accurately measure adiponectin in serum/plasma using any automatic chemical analyser.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of adiponectin in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with an antibody highly specific to adiponectin, is added into the cuvette and mixed. The presence of adiponectin in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of adiponectin in unknown samples can be interpolated from a reference curve using the standards provided.
